Protein_Domain
SP-Lipocal

Part:BBa_K157036

Designed by: iGEM Team Freiburg 2008   Group: iGEM08_Freiburg   (2008-10-26)

SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cCFP

A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor CFP with a fluolinker joined to its N-Terminus.
Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005.
For further information see the description of the parts mentioned above.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 335
    Illegal BamHI site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 1042
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 12


[edit]
Categories
//function/reporter/fluorescence
//proteindomain/internal
Parameters
None